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| Treatment | N | Vaccination | Challenge | Necropsy PCV-2 | Necropsy Mhyo |
|---|
| Left Side of the Neck (volume and route) | Right Side of the Neck (volume and route) |
|---|
| NV-NC | 6 | PBS (2.5 mL IM) | – | PBS | PCV-2 lesion assessment | Mhyo lung lesion scoring |
| NV-C/PCV-2 | 15 | PBS (2.5 mL IM) | – | PCV-2b | PCV-2 lesion assessment | – |
| VS-C/PCV-2 | 15 | Circovac (0.5 mL IM) | Hyogen (2.0 mL IM) | PCV-2b | – |
| VC-C/PCV-2 | 15 | Circovac + Hyogen (2.5 mL IM RTM) | – | PCV-2b | – |
| NV-C/Mhyo | 15 | PBS (2.5 mL IM) | – | Mhyo | – | Mhyo Lung lesion scoring |
| VS-C/Mhyo | 15 | Circovac (0.5 mL IM) | Hyogen (2.0 mL IM) | Mhyo | – |
| VC-C/Mhyo | 15 | Circovac + Hyogen (2.5 mL IM RTM) | – | Mhyo | – |
- NV-NC Non-vaccinated Non-challenged, NV-C Non-vaccinated-Challenged animals, VS-C Challenged (with PCV2 or Mhyo) Separate vaccination, VC-C (with PCV2 or Mhyo) Combined (RTM) vaccination
